The Resource Rapid peptide reagent isolation in a disposable microfluidic cartridge, Dimitra N. Stratis-Cullum, Joshua M. Kogot, and Paul M. Pellegrino, (electronic resource)

Rapid peptide reagent isolation in a disposable microfluidic cartridge, Dimitra N. Stratis-Cullum, Joshua M. Kogot, and Paul M. Pellegrino, (electronic resource)

Label
Rapid peptide reagent isolation in a disposable microfluidic cartridge
Title
Rapid peptide reagent isolation in a disposable microfluidic cartridge
Statement of responsibility
Dimitra N. Stratis-Cullum, Joshua M. Kogot, and Paul M. Pellegrino
Creator
Contributor
Subject
Language
eng
Summary
In this collaborative development project, the challenges in affinity reagent isolation were addressed by combining two seemingly disjoint technologies-biosynthetic display libraries and microfluidic systems. To isolate optimal ligands from these libraries, our project developed an automated magnetic bacterial cell sorting system, termed micromagnetic cell sorter (MMS), to screen microbial libraries with unprecedented throughput using a disposable microfluidic cartridge. We report on the sorting system performance in rare cell and ultra-rare cell recovery (populations less than 0.001%), and compare the results to the standard manual method, magnetic activated cell Sorting (MACS). To demonstrate the system effectiveness in reagent isolation through sorting against an anthrax toxin target, protective antigen was performed and comparable results to standard screening methods were obtained. In addition, the good recovery performance through MMS selection yielded a consensus sequence among 24 unique binders
Member of
Cataloging source
DTICE
http://library.link/vocab/creatorName
Stratis-Cullum, Dimitra N
Government publication
federal national government publication
Illustrations
illustrations
Index
no index present
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
  • technical reports
Period covered
June 2006-September 2009.
http://library.link/vocab/relatedWorkOrContributorName
  • Kogot, Joshua M
  • Pellegrino, Paul M
  • U.S. Army Research Laboratory
Series statement
ARL-TR
Series volume
5357
http://library.link/vocab/subjectName
  • Cytology
  • Peptides
  • Biological reagents
  • Biological weapons
  • Chemical, Biological and Radiological Warfare
  • Ammunition and Explosives
  • Grignard reagents
  • Cartridges
  • Isolation
  • Peptides
  • Population
  • Display systems
  • Ligands
  • Chemical agents
  • Standardization
  • Sorting
  • Binders
  • Anthrax
  • Antigens
  • Biosynthesis
  • Bacterial toxins
  • Bacillus anthracis
  • Targets
  • Cells
  • Recovery
  • Magnetic fields
  • Activation
Type of report
Final;
Label
Rapid peptide reagent isolation in a disposable microfluidic cartridge, Dimitra N. Stratis-Cullum, Joshua M. Kogot, and Paul M. Pellegrino, (electronic resource)
Link
http://purl.fdlp.gov/GPO/gpo17215
Instantiates
Publication
Note
  • Title from title screen (viewed on Jan. 4, 2012)
  • "September 2010."
Bibliography note
Includes bibliographical references (p. 17-19)
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Color
mixed
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Dimensions
unknown
Extent
1 online resource (viii, 22 p.)
Form of item
online
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other physical details
col. ill.
Specific material designation
remote
Label
Rapid peptide reagent isolation in a disposable microfluidic cartridge, Dimitra N. Stratis-Cullum, Joshua M. Kogot, and Paul M. Pellegrino, (electronic resource)
Link
http://purl.fdlp.gov/GPO/gpo17215
Publication
Note
  • Title from title screen (viewed on Jan. 4, 2012)
  • "September 2010."
Bibliography note
Includes bibliographical references (p. 17-19)
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Color
mixed
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Dimensions
unknown
Extent
1 online resource (viii, 22 p.)
Form of item
online
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other physical details
col. ill.
Specific material designation
remote

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